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PXD000176

PXD000176 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentifying RasGAP SH2 binding partners in Drosophila using mass spectrometry
DescriptionDrosophila S2 cells were transfected with RasGAP(WT), RasGAP(SH2*32*) and GFP control. 4 mg of S2 cell lysate was used to pulldown LAP and GFP tagged constructs using GFP-Trap. The pulled down fraction was loaded on SDS-PAGE gels and coomassie stained before several bands (a-u) from two independent but parallel experiments were excised and subjected to MS analysis.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_05:19:12.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000176
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBehzad Rowshanravan
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListiodoacetamide - site C: 57.021464; phosphorylated residue: 79.966331
InstrumentLTQ Orbitrap; instrument model: LTQ-Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-08 05:11:07ID requested
12014-05-20 01:24:26announced
22014-07-24 05:19:13announcedUpdated project metadata.
Publication List
Rowshanravan B, Woodcock SA, Botella JA, Kiermayer C, Schneuwly S, Hughes DA, RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis. J Cell Sci, 127(Pt 13):2849-61(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Drosophila, Schneider 2 (S2) cells, RasGAP, SH2
Contact List
Behzad Rowshanravan
contact affiliationFaculty of Life Sciences
contact emailbehr87@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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