PXD000176 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identifying RasGAP SH2 binding partners in Drosophila using mass spectrometry |
Description | Drosophila S2 cells were transfected with RasGAP(WT), RasGAP(SH2*32*) and GFP control. 4 mg of S2 cell lysate was used to pulldown LAP and GFP tagged constructs using GFP-Trap. The pulled down fraction was loaded on SDS-PAGE gels and coomassie stained before several bands (a-u) from two independent but parallel experiments were excised and subjected to MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_05:19:12.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000176 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Behzad Rowshanravan |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | iodoacetamide - site C: 57.021464; phosphorylated residue: 79.966331 |
Instrument | LTQ Orbitrap; instrument model: LTQ-Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-03-08 05:11:07 | ID requested | |
1 | 2014-05-20 01:24:26 | announced | |
⏵ 2 | 2014-07-24 05:19:13 | announced | Updated project metadata. |
Publication List
Rowshanravan B, Woodcock SA, Botella JA, Kiermayer C, Schneuwly S, Hughes DA, RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis. J Cell Sci, 127(Pt 13):2849-61(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Drosophila, Schneider 2 (S2) cells, RasGAP, SH2 |
Contact List
Behzad Rowshanravan |
contact affiliation | Faculty of Life Sciences |
contact email | behr87@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/05/PXD000176 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000176
- Label: PRIDE project
- Name: Identifying RasGAP SH2 binding partners in Drosophila using mass spectrometry