PXD000175 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis uncover a new function and mode of action for mDia2 |
Description | mDia2 is an auto-inhibited Formin influencing actin dynamics upon conversion to the active conformation. mDia2 regulates actin-based protrusions and cell invasion, cell differentiation, vesicle trafficking and cytokinesis. However, whether mDia2 has additional functions and how its action is functionally specified remain unknown. Here we draw the interactome of auto-inhibited and constitutively active mDia2 to address these issues. We embed mDia2 in protein networks accounting for its attributed functions and unexpectedly link it to the Ubiquitin Proteasome System. Taking FBXO3 as a test case, we show that mDia2 binds FBXO3 and p53, and regulates p53 transcriptional activity in an actin-nucleation-independent and conformation-insensitive manner. Increased mDia2 and FBXO3 levels elevate p53 activity and expression thereby sensitizing cells to p53-dependent apoptosis, whereas their decrease produces opposite effects. Thus, we discover a new role of mDia2 in p53 regulation suggesting that the closed conformation is biologically active and an FBXO3-based mechanism to functionally specify mDia2’s activity. |
HostingRepository | PRIDE |
AnnounceDate | 2015-01-06 |
AnnouncementXML | Submission_2015-01-06_08:08:30.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AFM Altelaar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-03-07 08:48:20 | ID requested | |
⏵ 1 | 2015-01-06 08:08:31 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Proteomics, AP-MS, mDia2 |
Contact List
Maarten Altelaar |
contact affiliation | Utrecht University |
contact email | m.altelaar@uu.nl |
lab head | |
AFM Altelaar |
contact affiliation | Faculty of Science |
contact email | m.altelaar@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000175
- Label: PRIDE project
- Name: Proteomic analysis uncover a new function and mode of action for mDia2