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PXD000175

PXD000175 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis uncover a new function and mode of action for mDia2
DescriptionmDia2 is an auto-inhibited Formin influencing actin dynamics upon conversion to the active conformation. mDia2 regulates actin-based protrusions and cell invasion, cell differentiation, vesicle trafficking and cytokinesis. However, whether mDia2 has additional functions and how its action is functionally specified remain unknown. Here we draw the interactome of auto-inhibited and constitutively active mDia2 to address these issues. We embed mDia2 in protein networks accounting for its attributed functions and unexpectedly link it to the Ubiquitin Proteasome System. Taking FBXO3 as a test case, we show that mDia2 binds FBXO3 and p53, and regulates p53 transcriptional activity in an actin-nucleation-independent and conformation-insensitive manner. Increased mDia2 and FBXO3 levels elevate p53 activity and expression thereby sensitizing cells to p53-dependent apoptosis, whereas their decrease produces opposite effects. Thus, we discover a new role of mDia2 in p53 regulation suggesting that the closed conformation is biologically active and an FBXO3-based mechanism to functionally specify mDia2’s activity.
HostingRepositoryPRIDE
AnnounceDate2015-01-06
AnnouncementXMLSubmission_2015-01-06_08:08:30.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAFM Altelaar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-07 08:48:20ID requested
12015-01-06 08:08:31announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Proteomics, AP-MS, mDia2
Contact List
Maarten Altelaar
contact affiliationUtrecht University
contact emailm.altelaar@uu.nl
lab head
AFM Altelaar
contact affiliationFaculty of Science
contact emailm.altelaar@uu.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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