PXD000170

PXD000170 is an original dataset announced via ProteomeXchange.

Title	Identifying sources of tick blood meals using unidentified tandem mass spectral libraries
Description	Rapid and reliable identification of the vertebrate species on which a disease vector previously parasitized is imperative to study ecological factors that affect pathogen distribution and can aid the development of public health programs. We here describe a proteome profiling technique designed to identify the source of blood meals of hematophagous arthropods. This method employs direct spectral matching and thus does not require a priori knowledge of any genetic or protein sequence information. Using this technology, we can detect remnants of blood in blacklegged ticks (Ixodes scapularis) and correctly determine the vertebrate species from which the blood was derived even six months after the tick had fed. This biological fingerprinting methodology is sensitive, fast, cost-effective, and can potentially be adapted for other biological and medical applications when existing genome-based methods are impractical or ineffective.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_00:56:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henry Lam
SpeciesList	scientific name: Panthera leo (Lion); NCBI TaxID: 9689; scientific name: Macropus rufus (Red kangaroo) (Megaleia rufa); NCBI TaxID: 9321; scientific name: Alligator mississippiensis (American alligator); NCBI TaxID: 8496; scientific name: Capra hircus (Goat); NCBI TaxID: 9925; scientific name: Colobus guereza (Mantled guereza) (Eastern black-and-white colobus monkey); NCBI TaxID: 33548; scientific name: Strix varia (Barred owl); NCBI TaxID: 57075; scientific name: Eryx colubrinus loveridgei; NCBI TaxID: 51866; scientific name: Sciurus carolinensis (Gray squirrel); NCBI TaxID: 30640; scientific name: Chelodina longicollis (Snake-necked turtle) (Testudo longicollis); NCBI TaxID: 44491; scientific name: Pteropus rodricensis (Rodriguez flying fox); NCBI TaxID: 77216; scientific name: Corucia zebrata; NCBI TaxID: 96731; scientific name: Anas platyrhynchos (Domestic duck) (Anas boschas); NCBI TaxID: 8839; scientific name: Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat); NCBI TaxID: 9407; scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945; scientific name: Tremarctos ornatus (Spectacled bear); NCBI TaxID: 9638; scientific name: Panthera tigris (Tiger); NCBI TaxID: 9694; scientific name: Nanger dama mhorr; NCBI TaxID: 261756; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Malaclemys terrapin (Diamondback terrapin) (Testudo terrapin); NCBI TaxID: 8485; scientific name: Rhynchocyon petersi (black and rufous elephant shrew); NCBI TaxID: 320637; scientific name: Sturnus vulgaris (Starling); NCBI TaxID: 9172; scientific name: Ovis aries (Sheep); NCBI TaxID: 9940; scientific name: Peromyscus leucopus (White-footed mouse); NCBI TaxID: 10041; scientific name: Equus asinus africanus; NCBI TaxID: 582580; scientific name: Pituophis melanoleucus (Pine snake) (Coluber melanoleucus); NCBI TaxID: 8595; scientific name: Tamias striatus (Eastern chipmunk); NCBI TaxID: 45474;
ModificationList	iodoacetamide derivatized residue
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2013-03-01 03:26:44	ID requested
1	2014-04-03 02:59:06	announced
⏵ 2	2014-07-25 00:56:41	announced	Updated project metadata.

Ö, nder Ö, Shao W, Kemps BD, Lam H, Brisson D, Identifying sources of tick blood meals using unidentified tandem mass spectral libraries. Nat Commun, 4():1746(2013) [pubmed]

submitter keyword: Unknown genome proteomics, ecological proteomics, spectral libraries

Henry Lam
contact affiliation	Hong Kong University of Science and Technology
contact email	kehlam@ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000170

PRIDE project URI

• PRIDE
  1. PXD000170
     1. Label: PRIDE project
     2. Name: Identifying sources of tick blood meals using unidentified tandem mass spectral libraries