PXD000169

PXD000169 is an original dataset announced via ProteomeXchange.

Title	Analysis of the honeybee sperm proteome using MudPit and 2D PAGE
Description	Honeybee semen was collected by gently squeezing the male abdomens. Samples were pooled and then centrifuged. Pelleted sperm was collected and analysed with both 2D PAGE and gel-free methods. For the 2D PAGE, protein spots were digested using trypsin. Extracted peptides, resolved on a C18 column, were analysed by an Agilent LC/MSD Trap XCT Ultra 6330 mass spectrometer. Spectra were searched against the honeybee protein sequences (RefSeq release 48) using Mascot algorithm (with 1 missed cleavage, Cys-carbamidomethylation as fixed modification, and Met-oxidation and N/Q-deamidation as variable modifications). For the MudPit analysis the sperm sample was digested by trypsin. Peptides were resolved using strong cation exchange chromatography followed by reverse phase (C18) HPLC and finally analysed by an Agilent QTOF mass spectrometer. Resulting spectrum files were converted to mzXML and merged using ProteoWizard msconvert. These were then searched against honeybee protein sequences using Mascot, Omssa and X!tandem (all with 1 missed cleavage, and Met-oxidation and N/Q-deamidation as variable modifications). Results of the three search engines were pooled using TPP.
HostingRepository	PRIDE
AnnounceDate	2020-01-17
AnnouncementXML	Submission_2020-01-17_01:17:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Reza Zareie
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; deamidated residue
Instrument	6510 Quadrupole Time-of-Flight LC/MS; 6330 Ion Trap LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2013-03-01 01:53:01	ID requested
⏵ 1	2020-01-17 01:17:11	announced

Zareie R, Eubel H, Millar AH, Baer B, Long-term survival of high quality sperm: insights into the sperm proteome of the honeybee Apis mellifera. J Proteome Res, 12(11):5180-8(2013) [pubmed]

submitter keyword: Honey bee, sperm. LC-MS, MudPit, 2D PAGE

Boris Baer
contact affiliation	†Centre for Integrative Bee Research (CIBER), ARC Centre of Excellence in Plant Energy Biology, ‡ Centre for Comparative Analysis of Biomolecular Networks, and ⊥Centre for Evolutionary Biology, School of Animal Biology, The University of Western Australia, 6009 Crawley, Australia
contact email	boris.baer@uwa.edu.au
lab head
Reza Zareie
contact affiliation	University of Western Australia
contact email	reza.zareie@uwa.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000169
     1. Label: PRIDE project
     2. Name: Analysis of the honeybee sperm proteome using MudPit and 2D PAGE