PXD000155

PXD000155 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of integrin activation-state-dependent adhesion complexes
Description	Proteomic analysis of integrin activation-state-dependent adhesion complexes. Adhesion complexes were isolated from K562 cells using activation-state-specific monoclonal antibodies coupled to magnetic beads.Tandem mass spectra were extracted using extract_msn (Thermo Fisher Scientific) executed in Mascot Daemon (version 2.2.2; Matrix Science). Peak list files were searched against the IPI Human database (version 3.70) modified to contain ten additional contaminants and reagent sequences of non-human origin. Searches were submitted to an in-house Mascot server (version 2.2.03; Matrix Science). Carbamidomethylation of cysteine was set as a fixed modification, and oxidation of methionine was allowed as a variable modification. Only tryptic peptides were considered, with up to one missed cleavage permitted. Monoisotopic precursor mass values were used, and only doubly and triply charged precursor ions were considered. Mass tolerances for precursor and fragment ions were 0.4 Da and 0.5 Da, respectively.
HostingRepository	PRIDE
AnnounceDate	2015-01-31
AnnouncementXML	Submission_2015-01-31_00:30:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000155
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adam Byron
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ Velos; LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-02-21 01:31:11	ID requested
1	2015-01-27 05:11:11	announced
⏵ 2	2015-01-31 00:30:39	announced	Updated project metadata.

Byron A, Askari JA, Humphries JD, Jacquemet G, Koper EJ, Warwood S, Choi CK, Stroud MJ, Chen CS, Knight D, Humphries MJ, A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nat Commun, 6():6135(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, K562, Integrin, Activation State, Adhesion Complex, Proteomics, MS

Adam Byron
contact affiliation	Faculty of Life Sciences
contact email	adam.byron@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD000155

PRIDE project URI

• PRIDE
  1. PXD000155
     1. Label: PRIDE project
     2. Name: Proteomic analysis of integrin activation-state-dependent adhesion complexes