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PXD000152

PXD000152 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConservation of the extended substrate specificity profiles among homologous granzymes across species
DescriptionGranzymes are a family of structurally related serine proteases involved in immunity. To date four out of five human granzymes have been assigned orthologues in mice; however for granzyme H, no clear murine orthologue has been suggested and its role in cytotoxicity remains controversial. In this study, we demonstrate that granzyme H is an inefficient cytotoxin. Besides analyzing the substrate specificity profile of granzyme H by means of substrate phage display, we assessed human granzyme H and mouse granzyme C cleavage susceptibility on a proteome-wide level. The extended specificity profiles of granzyme C and granzyme H (i.e. beyond P4-P4') match those previously observed for granzyme B. We demonstrate conservation of these extended specificity profiles among various granzymes since granzyme B cleavage susceptibility of an otherwise granzyme H/C specific cleavage site can be conferred simply by altering the P1-residue to aspartate, the preferred P1-residue of granzyme B. Our results thus indicate a conserved, but hitherto generally underappreciated specificity-determining role of extended protease-substrate contacts in steering cleavage susceptibility. Bio-IT: The spectra were searched with Mascot Daemon 2.2. The following search parameters were used. Peptide mass tolerance was set at 0.2 Da and peptide fragment mass tolerance at 0.1 Da; with the ESI-QUAD-TOF as selected instrument for peptide fragmentation rules for the Q-TOF Premier data. Endoproteinase semiArg-C/P (i.e., no restriction towards arginine-proline cleavage) was set as enzyme allowing one missed cleavage. Variable modifications were pyroglutamate formation of N-terminal glutamine, pyrocarbamidomethyl formation of N-terminal alkylated cysteine, deamidation of asparagine, acetylation or tri-deuteroacetylation of the alpha-N-terminus. Fixed modifications were methionine oxidation (sulfoxide), carbamidomethyl for cysteine, tri-deuteroacetylation of lysine and for identifying heavy labelled peptides, [13C6] or [13C615N4]-arginine were additionally set as fixed modifications. The spectra and identifications were stored in ms_lims
HostingRepositoryPRIDE
AnnounceDate2014-08-08
AnnouncementXMLSubmission_2014-08-08_00:30:44.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000152
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPieter-Jan De Bock
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListL-methionine sulfoxide: 15.994915; 6x(13)C: 4x(15)N labeled L-arginine; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; 6x(13)C labeled L-arginine: 6.020129; iodoacetamide derivatized residue: 57.021464; N-acetylated residue: 42.010565; deamidated residue: 0.984016; acetate labeling reagent (N-term) (heavy form: +3amu)
Instrumentinstrument model: QToF Global; Waters instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-02-19 08:03:24ID requested
12013-11-27 02:43:48announced
12013-11-27 02:52:20announced
22014-07-28 00:29:57announcedUpdated project metadata.
32014-08-08 00:30:45announcedUpdated project metadata.
Publication List
Plasman K, Maurer-Stroh S, Ahmad J, Hao H, Kaiserman D, Sirota FL, Jonckheere V, Bird PI, Gevaert K, Van Damme P, Conservation of the extended substrate specificity profiles among homologous granzymes across species. Mol Cell Proteomics, 12(10):2921-34(2013) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Granzyme H, granzyme C, human, mouse, LC-MSMS, N-terminal Cofradic
Contact List
Pieter-Jan De Bock
contact affiliationBiochemistry
contact emailpieter-jan.debock@cea.fr
dataset submitter
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Dataset FTP location
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