PXD000150

PXD000150 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MSE vs IM-MSE comparison
Description	The experiment was conducted to compare the MSE and IM-MSE acquisiton modes as well as to find the optimal on column loading. This was done using 1DLC and cytosolic e coli digest.Raw data were processed and searched using Proteinlynx Global Server version 3.0. The following parameters were used to generate peak lists: minimum intensity for precursors was set to 140, minimum intensity for fragment ions 30 and total bin-count was set to minimum of 400. Processed data was searched against the UniprotKB, Escherichia coli K12 strain, concatenated with 125 common laboratory contaminates. Fixed modification was set to carbamidomethylation of C and variable modification was set to oxidation of M. Searches were conducted against a froward and reverse sequence database. Minimum identification criteria included 3 fragment ions per peptide, 5 fragment ions per protein, minimum peptide identification score of 5.9 and minimum of 2 peptides per protein. Search results and spectra were imported into Scaffold and the global false discovery rate was less than 1%.
HostingRepository	PRIDE
AnnounceDate	2013-02-18
AnnouncementXML	Submission_2013-02-18_03:05:40.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue; monohydroxylated residue
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status
0	2013-02-18 02:35:24	ID requested
⏵ 1	2013-02-18 03:05:40	announced
⏵ 1	2014-07-25 00:54:01	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: MSE, e coli, LC-MS/MS

submitter keyword: MSE, e coli, LC-MS/MS

Contact List

Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel National Center for Personalized Medicine
contact email	yishai.levin@gmail.com

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/02/PXD000150

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/02/PXD000150