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PXD000134

PXD000134 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells
DescriptionAssessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature. See the Data Processing section of the published paper concerning the bioinformatics pipeline used. PMCID: PMC3261715 PMID: 22108792 Mol Syst Biol. 2011 Nov 22;7:550. doi: 10.1038/msb.2011.84. The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Munoz J, Low TY, Kok YJ, Chin A, Frese CK, Ding V, Choo A, Heck AJ. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
HostingRepositoryPRIDE
AnnounceDate2013-02-01
AnnouncementXMLSubmission_2013-02-01_04:04:10.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTeck Yew Low
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue; monohydroxylated residue
InstrumentLCQ Classic; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-01-30 08:36:06ID requested
12013-02-01 03:59:55announced
12014-07-25 00:51:22announced
22013-02-01 04:04:10announcedAdd reference
Publication List
Munoz J, Low TY, Kok YJ, Chin A, Frese CK, Ding V, Choo A, Heck AJ, The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Mol Syst Biol, 7():550(2011) [pubmed]
Keyword List
submitter keyword: iPS cells, embryonic stem cells, fibroblast, reprogramming, LC-MSMS, ETD
Contact List
Teck Yew Low
contact affiliationUtrecht Univeristy
contact emaillowteckyew@gmail.com
Full Dataset Link List
Dataset FTP location
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