PXD000128

PXD000128 is an original dataset announced via ProteomeXchange.

Title	Rat Liver Microsomes by 2D-LC-MS/MS
Description	Rat liver microsomes were analysed using offline strong cation exchange fractionation followed by reverse-phase chromatography coupled to quadrupole-time-of-flight high-resolution mass spectrometry using trypsin and pepsin parallel digestions. MS/MS files were searched against the UniProt protein database (release date 12-07-2011, 241 MB) by ProteinPilot software (version 4.1) using Paragon algorithm with the following parameters: Protein identification with thorough ID search effort, iodoacetamide for cysteine alkylation and no specified proteolytic enzyme, species set for rattus norvegicus with ID focus on biological modifications. Detection protein threshold of unused ProtScore > 0.05 (confidence > 10.0%) with false discovery rate (FDR) analysis. MS tolerance 0.05 Da on precursor ions and 0.1 Da on fragments. Search was performed for +2 to +4 charge states.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-08-08_02:09:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lekha Sleno
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2013-01-22 03:50:29	ID requested
1	2013-02-19 01:35:25	announced
1	2014-07-25 00:50:38	announced
⏵ 2	2014-08-08 02:09:37	announced	Updated publication reference for PubMed record(s): 23454493.

Golizeh M, Sleno L, Optimized proteomic analysis of rat liver microsomes using dual enzyme digestion with 2D-LC-MS/MS. J Proteomics, 82():166-78(2013) [pubmed]

submitter keyword: Rat, Liver, Microsomes, trypsin, pepsin, Strong Cation Exchange, QqTOF

Lekha Sleno
contact affiliation	Chemistry
contact email	sleno.lekha@uqam.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/02/PXD000128

PRIDE project URI

• PRIDE
  1. PXD000128
     1. Label: PRIDE project
     2. Name: Rat Liver Microsomes by 2D-LC-MS/MS