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PXD000119

PXD000119 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNano HPLC Chip Ion Trap MS/MS of human pancreatic cancer cells
DescriptionProteins extracted from Panc1 cells (untreated or treated with ACPA or GW), were analyzed by 2-DE. Modulated proteins were identified by nanoHPLC Chip Ion trap. Bioinformatics pipeline: Mascot search was applied using the MS/MS ion search of Mascot against human entries of the non-redundant NCBI database, with propionamide formation of cysteines as fixed modification and oxidized methionine, acetylated protein N terminus, and phosphorylation of serine, threonine, and tyrosine as variable modifications. Trypsin was specified as the proteolytic enzyme and one missed cleavage was allowed. The mass tolerance of the precursor and fragment ions was set to +- 0.9 Da.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_08:47:11.554.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000119
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDaniela Cecconi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidoethyl-L-cysteine; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrumentesquire 6000
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-01-10 01:30:14ID requested
12014-07-10 03:12:24announced
22014-07-24 03:46:56announcedUpdated project metadata.
32024-10-07 08:47:12announced2024-10-07: Updated project metadata.
Publication List
10.6019/PXD000119;
10.1002/elps.201200402;
Brandi J, Dando I, Palmieri M, Donadelli M, Cecconi D, Comparative proteomic and phosphoproteomic profiling of pancreatic adenocarcinoma cells treated with CB1 or CB2 agonists. Electrophoresis, 34(9-10):1359-68(2013) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, LC MS/MS, Panc1 cells
Contact List
Daniela Cecconi
contact affiliationUniversity of Verona
contact emaildaniela.cecconi@univr.it
lab head
Daniela Cecconi
contact affiliationUniversity of Verona
contact emaildaniela.cecconi@univr.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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