PXD000119

PXD000119 is an original dataset announced via ProteomeXchange.

Title	Nano HPLC Chip Ion Trap MS/MS of human pancreatic cancer cells
Description	Proteins extracted from Panc1 cells (untreated or treated with ACPA or GW), were analyzed by 2-DE. Modulated proteins were identified by nanoHPLC Chip Ion trap. Bioinformatics pipeline: Mascot search was applied using the MS/MS ion search of Mascot against human entries of the non-redundant NCBI database, with propionamide formation of cysteines as fixed modification and oxidized methionine, acetylated protein N terminus, and phosphorylation of serine, threonine, and tyrosine as variable modifications. Trypsin was specified as the proteolytic enzyme and one missed cleavage was allowed. The mass tolerance of the precursor and fragment ions was set to +- 0.9 Da.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:46:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000119
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Cecconi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue; phosphorylated residue
Instrument	instrument model: nano HPLC Chip Ion Trap

Revision	Datetime	Status	ChangeLog Entry
0	2013-01-10 01:30:14	ID requested
1	2014-07-10 03:12:24	announced
⏵ 2	2014-07-24 03:46:56	announced	Updated project metadata.

Brandi J, Dando I, Palmieri M, Donadelli M, Cecconi D, Comparative proteomic and phosphoproteomic profiling of pancreatic adenocarcinoma cells treated with CB1 or CB2 agonists. Electrophoresis, 34(9-10):1359-68(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Panc1 cells, LC MS/MS

Daniela Cecconi
contact affiliation	Department of Biotechnology
contact email	daniela.cecconi@univr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000119

PRIDE project URI

• PRIDE
  1. PXD000119
     1. Label: PRIDE project
     2. Name: Nano HPLC Chip Ion Trap MS/MS of human pancreatic cancer cells