PXD000117

PXD000117 is an original dataset announced via ProteomeXchange.

Title	Human exosome proteome
Description	Proteomics study of human urine exosomes. This data describes the proteomic complement of the human exosomal urine fraction from 10 healthy volunteers (5 male, 5 female) aged 23 to 36 years. 50 µg protein from each sample were fragmented on a 4%/12% SDS-polyacrylamide gel. Following staining, each gel track was separated into 28 equal sections, which were processed individually. Peptides were separated by reverse-phase chromatography (Dionex, Sunnyvale, CA) and LC-MS/MS was performed using an Eksigent NanoLC-1D Plus (Eksigent Technologies, Dublin, CA) HPLC system and an LTQ Orbitrap mass spectrometer (ThermoFisher, Waltham, MA). Peptides from each gel segment were analysed twice: (1) with a dynamic exclusion list and (2) a fixed exclusion list for the abundant protein uromodulin which was superimposed on a dynamic exclusion. MS data were processed using SEQUEST Bioworks Browser (version 3.3.1 SP1, ThermoFisher) to generate MS/MS peak lists. Combined peak list files were submitted to the MASCOT search algorithm (version 2.2.1, Matrix Science, London UK) and searched against the IPI-Human database.
HostingRepository	PRIDE
AnnounceDate	2016-08-25
AnnouncementXML	Submission_2016-08-25_04:54:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000117
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laurent Gatto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ Orbitrap; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-01-03 02:07:42	ID requested
⏵ 1	2016-08-25 04:54:56	announced

Hiemstra TF, Charles PD, Gracia T, Hester SS, Gatto L, Al-Lamki R, Floto RA, Su Y, Skepper JN, Lilley KS, Karet Frankl FE, Human urinary exosomes as innate immune effectors. J Am Soc Nephrol, 25(9):2017-27(2014) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

submitter keyword: human, proteome, exosome, urine

Kathryn Lilley
contact affiliation	University of Cambridge
contact email	ksl23@cam.ac.uk
lab head
Laurent Gatto
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	lg390@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD000117

PRIDE project URI

• PRIDE
  1. PXD000117
     1. Label: PRIDE project
     2. Name: Human exosome proteome