PXD000115 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Npro protein interaction partners |
Description | The N-terminal protease Npro from pestiviruses has been shown to rapidly dismantle the innate immune response by targeting IRF3 for degradation, resulting in inhibition of apoptosis and interferon production. To understand it’s role in these different responses, we have identified multiple cellular factors that interact with this viral protein using mass spectrometry and proteomic analysis. Pull-down experiments were performed with Npro, and the samples were run on an SDS gel. Each lane was cut into 5-6 sclices and the slices were digested with trypsin. Peptides were extracted and analysed by LX-MSMS on an LTQ-Orbitrap (Thermo). Data were processed with MaxQuant (1.3.0.5) and searches performed with Mascot on the Sptrembl database (taxonomy human). All gel slices from one sample were merged in one Mascot search. Mascot results were imported into Scaffold 3.6.5. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_08:46:31.013.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000115 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gerhard Saalbach |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-01-02 03:05:14 | ID requested | |
1 | 2016-12-22 07:50:19 | announced | |
2 | 2016-12-23 00:30:24 | announced | Updated project metadata. |
3 | 2017-10-24 03:19:26 | announced | Updated project metadata. |
⏵ 4 | 2024-10-07 08:46:31 | announced | 2024-10-07: Updated project metadata. |
Publication List
10.1128/JVI.00984-14; |
10.6019/PXD000115; |
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP, Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex. J Virol, 88(18):10340-53(2014) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: LC-MSMS, protein interaction, human,Pestivirus N-terminal Protease Npro |
Contact List
Dr. Penny Powell |
contact affiliation | Senior Lecturer, Norwich Medical School, University of East Anglia UEA |
contact email | p.powell@uea.ac.uk |
lab head | |
Gerhard Saalbach |
contact affiliation | Biological Chemistry |
contact email | gerhard.saalbach@jic.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000115
- Label: PRIDE project
- Name: Npro protein interaction partners