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PXD000115

PXD000115 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNpro protein interaction partners
DescriptionThe N-terminal protease Npro from pestiviruses has been shown to rapidly dismantle the innate immune response by targeting IRF3 for degradation, resulting in inhibition of apoptosis and interferon production. To understand it’s role in these different responses, we have identified multiple cellular factors that interact with this viral protein using mass spectrometry and proteomic analysis. Pull-down experiments were performed with Npro, and the samples were run on an SDS gel. Each lane was cut into 5-6 sclices and the slices were digested with trypsin. Peptides were extracted and analysed by LX-MSMS on an LTQ-Orbitrap (Thermo). Data were processed with MaxQuant (1.3.0.5) and searches performed with Mascot on the Sptrembl database (taxonomy human). All gel slices from one sample were merged in one Mascot search. Mascot results were imported into Scaffold 3.6.5.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_08:46:31.013.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000115
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGerhard Saalbach
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-01-02 03:05:14ID requested
12016-12-22 07:50:19announced
22016-12-23 00:30:24announcedUpdated project metadata.
32017-10-24 03:19:26announcedUpdated project metadata.
42024-10-07 08:46:31announced2024-10-07: Updated project metadata.
Publication List
10.1128/JVI.00984-14;
10.6019/PXD000115;
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP, Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex. J Virol, 88(18):10340-53(2014) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: LC-MSMS, protein interaction, human,Pestivirus N-terminal Protease Npro
Contact List
Dr. Penny Powell
contact affiliationSenior Lecturer, Norwich Medical School, University of East Anglia UEA
contact emailp.powell@uea.ac.uk
lab head
Gerhard Saalbach
contact affiliationBiological Chemistry
contact emailgerhard.saalbach@jic.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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