PXD000115

PXD000115 is an original dataset announced via ProteomeXchange.

Title	Npro protein interaction partners
Description	The N-terminal protease Npro from pestiviruses has been shown to rapidly dismantle the innate immune response by targeting IRF3 for degradation, resulting in inhibition of apoptosis and interferon production. To understand it’s role in these different responses, we have identified multiple cellular factors that interact with this viral protein using mass spectrometry and proteomic analysis. Pull-down experiments were performed with Npro, and the samples were run on an SDS gel. Each lane was cut into 5-6 sclices and the slices were digested with trypsin. Peptides were extracted and analysed by LX-MSMS on an LTQ-Orbitrap (Thermo). Data were processed with MaxQuant (1.3.0.5) and searches performed with Mascot on the Sptrembl database (taxonomy human). All gel slices from one sample were merged in one Mascot search. Mascot results were imported into Scaffold 3.6.5.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_08:46:31.013.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000115
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-01-02 03:05:14	ID requested
1	2016-12-22 07:50:19	announced
2	2016-12-23 00:30:24	announced	Updated project metadata.
3	2017-10-24 03:19:26	announced	Updated project metadata.
⏵ 4	2024-10-07 08:46:31	announced	2024-10-07: Updated project metadata.

10.1128/JVI.00984-14;

10.6019/PXD000115;

Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP, Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex. J Virol, 88(18):10340-53(2014) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: LC-MSMS, protein interaction, human,Pestivirus N-terminal Protease Npro

Dr. Penny Powell
contact affiliation	Senior Lecturer, Norwich Medical School, University of East Anglia UEA
contact email	p.powell@uea.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000115
     1. Label: PRIDE project
     2. Name: Npro protein interaction partners