PXD000113

PXD000113 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	GeLC-MS/MS analysis of cereal aphid saliva
Description	Saliva was collected from c.40,000 diet reared aphids, pooled and concentrated. Samples were coming from 2 aphid species: Metopolophium dirhodum and Sitobion avenae. Proteins were fractionated using SDS PAGE and visible bands were subjected to in gel digestion. Tryptic peptides were analysised on a Thermo LTQ-FT. Protein identification from the MS/MS data was performed using the TurboSEQUEST algorithm in BioWorks v. 3.2 (Thermo Fisher Scientific) to correlate the data against ACYPIproteins v2.1, the official protein set of the pea aphid genome assembly (33291 predicted protein models; accessed November 2011) available at http://www.aphidbase.com/aphidbase/downloads. The following search parameters were used: precursor-ion mass tolerance of 1.5 Da, fragment ion tolerance of 1.0 Da with methionine oxidation and cysteine carboxyamidomethylation specified as differential modifications and a maximum of two missed cleavage sites allowed. Two filters were applied: XCorr vs. charge state (1, 2, 3 and 4 = 1.50, 2.00, 2.50 and 3.00 respectively) and peptide probability (p<0.001). Matches with multiple unique peptides and a cumulative XCorr >20 are reported. PLoS One accepted: Proteomic profiling of cereal aphid saliva reveals ubiquitous and adaptative secreted proteins. Rao S.A.K., Carolan, J.C. and Wilkinson, T.L.W.
HostingRepository	PRIDE
AnnounceDate	2013-04-09
AnnouncementXML	Submission_2013-04-09_02:41:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Metopolophium dirhodum (Rose grain aphid); NCBI TaxID: 44670; scientific name: Sitobion avenae (English grain aphid); NCBI TaxID: 44664;
ModificationList	iodoacetic acid derivatized residue; monohydroxylated residue
Instrument	LTQ

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-18 08:54:20	ID requested
1	2013-04-09 02:38:52	announced
1	2014-07-25 00:48:49	announced
⏵ 2	2013-04-09 02:41:26	announced	Add reference

Publication List

Rao SA, Carolan JC, Wilkinson TL, Proteomic profiling of cereal aphid saliva reveals both ubiquitous and adaptive secreted proteins. PLoS One, 8(2):e57413(2013) [pubmed]

Rao SA, Carolan JC, Wilkinson TL, Proteomic profiling of cereal aphid saliva reveals both ubiquitous and adaptive secreted proteins. PLoS One, 8(2):e57413(2013) [pubmed]

Keyword List

submitter keyword: Aphids, Saliva, LTQ-FT, Metopolophium dirhodum , Sitobion avenae

submitter keyword: Aphids, Saliva, LTQ-FT, Metopolophium dirhodum , Sitobion avenae

Contact List

James Carolan
contact affiliation	NUI Maynooth Biology
contact email	james.carolan@nuim.ie

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/04/PXD000113

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/04/PXD000113