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PXD000111

PXD000111 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMycobacterium tuberculosis culture filtrate LC-MS/MS
DescriptionTriplicate culture filtrate samples of normal (log phase) and PBS cultured (starvation) M. tuberculosis were separated by SDS-PAGE, and 10 bands were excised from each lane after staining with Coomassie R-250. In-gel trypsin digestion was performed on gel bands followed by nanoLC. Eluting peptides were then analyzed on an LTQ FT instrument, and the resulting raw files were searched with Sequest (part of Proteome Discoverer) against an M. tuberculosis H37Rv database (downloaded from the Sanger Centre). The final combined dataset comprised 12,399 unique peptides derived from 1,362 proteins, each identified by a minimum of two distinct peptides.
HostingRepositoryPRIDE
AnnounceDate2017-10-24
AnnouncementXMLSubmission_2017-10-24_00:11:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
Reprocessed datasets that are derived from this datasetRPXD000650
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIda Rosenkrands
SpeciesList scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ FT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-12-18 04:25:37ID requested
12013-01-24 03:55:52announced
12014-07-25 00:47:59announced
22013-02-11 02:26:23announcedAdd reference
22017-06-21 00:30:34announcedUpdated project metadata.
32017-10-24 00:11:24announcedUpdated project metadata.
Publication List
Albrethsen J, Agner J, Piersma SR, H, ΓΈ, jrup P, Pham TV, Weldingh K, Jimenez CR, Andersen P, Rosenkrands I, Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems. Mol Cell Proteomics, 12(5):1180-91(2013) [pubmed]
Keyword List
submitter keyword: Tuberculosis, LC-MS/MS, culture filtrate
Contact List
Ida Rosenkrands
contact affiliationInfectious Disease Immunology
contact emailidr@ssi.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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