PXD000106

PXD000106 is an original dataset announced via ProteomeXchange.

Title	Chemical Proteomics Partners identification of Cycloamphilectene
Description	The identification of the cycloamphilectene cellular interactome has been performed, through chemical proteomics, to unambiguously define its mechanism of action and thus to understand its pharmacological effect. To achieve this goal, a small bioactive molecule (cycloamphilectene) linked to a matrix through a spacer arm “fishes out” its specific interactors from a cell lysate or a tissue extract. Once eluted, cellular partners are separated by SDS-PAGE and identified by high resolution MS and bioinformatics analysis . MS and MS/MS data were acquired using a Q-TOF Premier mass spectrometer (Waters Corp., Micromass, Manchester, United Kingdom). The five most intense ions were automatically chosen by the MassLynx (4.1) software and fragmented. After mass spectrometric measurements, data were automatically processed by ProteinLynx Global Server software to generate peak lists for protein identifications. Database searches were carried out with MASCOT server. The SwissProt human database (release 2010_11 of 02 Nov 10, 522019 sequences, 184241123 residues) was searched, allowing 2 missed cleavages, carbamidomethyl (C) as fixed modification o and oxidation (M) and phosphorylation (ST) as variable modifications. The peptide tolerance was set to 80 ppm and the MS/MS tolerance to 0.8 Da. The Raw data reported herein contains 4 different chemical proteomics experiments carried out to identify cycloamphilectene cellular partner.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_04:24:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000106
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luigi Margarucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-12 03:58:24	ID requested
1	2018-11-26 07:53:33	announced
⏵ 2	2018-12-04 04:24:33	announced	Updated project metadata.

Margarucci L, Monti MC, Esposito R, Tosco A, Hamel E, Riccio R, Casapullo A, N-Formyl-7-amino-11-cycloamphilectene, a marine sponge metabolite, binds to tubulin and modulates microtubule depolymerization. Mol Biosyst, 10(4):862-7(2014) [pubmed]

curator keyword: Biological

submitter keyword: Chemical Proteomics, Natural Products, Mass Spectrometry, Targets Identification

Prof. Agostino Casapullo
contact affiliation	Bio-organic Chemistry Lab, Department of Pharmacy, University of Salerno, via Giovanni Paolo II 132, 84084 Fisciano, Italy
contact email	casapullo@unisa.it
lab head
Luigi Margarucci
contact affiliation	Pharmaceutical Sciences
contact email	lmargarucci@unisa.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000106
     1. Label: PRIDE project
     2. Name: Chemical Proteomics Partners identification of Cycloamphilectene