PXD000098
PXD000098 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Label-free quantification of Leptospira (Ludwig et al.) |
| Description | Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 cell culture. Three different biological conditions were investigated: 1) a control sample grown at 30°C in exponential growth phase; 2) a sample treated in exponential growth phase for 12h with the antibiotic Ciprofloxacin (5 μg/mL) and 3) a sample treated in exponential growth phase for 24h with Ciprofloxacin (5 μg/mL). To assure comparability of protein abundances across the three conditions, the total protein content of each sample was determined by a bicinchoninic acid (BCA) protein assay, and an additional normalization step was performed based on the determined absolute concentrations of the anchor point proteins after data acquisition. |
| HostingRepository | PeptideAtlas |
| AnnounceDate | 2014-03-11 |
| AnnouncementXML | Submission_2014-03-12_13:28:15.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Christina Ludwig |
| SpeciesList | scientific name: Leptospira interrogans; NCBI TaxID: 173; |
| ModificationList | S-carboxamidomethyl-L-cysteine |
| Instrument | TSQ Vantage |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2012-12-11 16:32:24 | ID requested | |
| 1 | 2012-12-11 16:32:59 | announced | |
| 2 | 2012-12-11 17:04:44 | announced | missing |
| 3 | 2012-12-12 15:00:09 | announced | missing |
| ⏵ 4 | 2014-03-12 13:28:17 | announced | update LabHead |
Publication List
| Ludwig C, Claassen M, Schmidt A, Aebersold R, Estimation of absolute protein quantities of unlabeled samples by selected reaction monitoring mass spectrometry. Mol Cell Proteomics, 11(3):M111.013987(2012) [pubmed] |
Keyword List
| curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
| Christina Ludwig | |
|---|---|
| contact affiliation | IMSB |
| contact email | ludwig@imsb.biol.ethz.ch |
| dataset submitter | |
| Ruedi Aebersold | |
| contact affiliation | IMSB |
| contact email | aebersold@imsb.biol.ethz.ch |
| lab head | |
Full Dataset Link List
| PeptideAtlas dataset URI |
| PASSEL experiment URI |
| PASSEL transition group browser URI |
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