Developing Dynamic MRM Method for Agilent MassHunter for
proteins Q6PI98, Q8N2U9, Q96DM3, Q9H3N8 for researching proteome of 18th
chromosome. Normal human liver tissue extracted proteins lysis in HFIP 20%;
after reduction, alkylation and sigestion with trypsin, final
concentration 1 ug/ul, original concentration 12.6 ug/ul.
Normal human plasma lysis in HFIP 20% after immunodepletion on Hu-14
column, reduction, alkylation and digestion with trypsin; the original
concentration befor immunodepletion 53.17 mg/ml, after - 24.89 mg/ml
(concentrated); in sample - 1 mg/ml.