PXD000097
PXD000097 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | dMRM four human chr18 proteins in liver & plasma |
Description | Developing Dynamic MRM Method for Agilent MassHunter for proteins Q6PI98, Q8N2U9, Q96DM3, Q9H3N8 for researching proteome of 18th chromosome. Normal human liver tissue extracted proteins lysis in HFIP 20%; after reduction, alkylation and sigestion with trypsin, final concentration 1 ug/ul, original concentration 12.6 ug/ul. Normal human plasma lysis in HFIP 20% after immunodepletion on Hu-14 column, reduction, alkylation and digestion with trypsin; the original concentration befor immunodepletion 53.17 mg/ml, after - 24.89 mg/ml (concentrated); in sample - 1 mg/ml. |
HostingRepository | PeptideAtlas |
AnnounceDate | 2014-03-19 |
AnnouncementXML | Submission_2014-03-19_09:48:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Aleksey Filimonov |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue; L-selenocysteine (Cys) |
Instrument | 6460 Triple Quadrupole LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2012-12-11 16:29:33 | ID requested | |
1 | 2012-12-11 16:30:05 | announced | |
2 | 2012-12-11 17:05:11 | announced | missing |
⏵ 3 | 2014-03-19 09:48:24 | announced | update labhead |
Publication List
Zgoda VG, Kopylov AT, Tikhonova OV, Moisa AA, Pyndyk NV, Farafonova TE, Novikova SE, Lisitsa AV, Ponomarenko EA, Poverennaya EV, Radko SP, Khmeleva SA, Kurbatov LK, Filimonov AD, Bogolyubova NA, Ilgisonis EV, Chernobrovkin AL, Ivanov AS, Medvedev AE, Mezentsev YV, Moshkovskii SA, Naryzhny SN, Ilina EN, Kostrjukova ES, Alexeev DG, Tyakht AV, Govorun VM, Archakov AI, Chromosome 18 transcriptome profiling and targeted proteome mapping in depleted plasma, liver tissue and HepG2 cells. J Proteome Res, 12(1):123-34(2013) [pubmed] |
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
Aleksey Filimonov | |
---|---|
contact affiliation | IMSB |
contact email | filimonov.a.d@gmail.com |
dataset submitter | |
Sergei Moshkovskii | |
contact affiliation | IMSB |
contact email | sergei.moshkovskii@ibmh.msk.ru |
lab head |
Full Dataset Link List
PeptideAtlas dataset URI |
PASSEL experiment URI |
PASSEL transition group browser URI |