To discover new miRNA targets, we generated a C. elegans transgenic
line expressing a functional, N-terminally tandem affinity
purification (TAP)-tagged ALG-1 protein (TAP::ALG-1 strain) (see
Methods for details).We next purified TAP::ALG-1 complexes from
mixed-stage TAP::ALG-1 transgenic and wild-type animals (serving
as a mock control) and hybridized the associated mRNAs to twocolor
microarrays.