PXD000090

PXD000090 is an original dataset announced via ProteomeXchange.

Title	Bombyx mori anterior silk gland LC-MS/MS
Description	The anterior silk gland in the silkworm plays an important role in the process of liquid fibroin to solid silk fiber .In view of this,the proteomics analysis was applied to to study the relationship between the function of proteins in the anterior silk gland and the mechanism of spinning. The anterior silk glands on the 3rd day of fifth instar were dissected.Aftter 1D SDS-PAGE ,one gel lane was cut into 10 bands and each band further sliced into small pieces was subjected to in-gel tryptic digestion for 20 hours.The digested peptides were separated by RP nanoscale capillary liquid chromatography and analyzed using a surveyor LC system (Thermo Figgigan, San Jose, CA).The eluate from the RP column was analyzed by Finnigan LTQ(Thermo Electron Corporation）linear ion trap Mass equipped with a nanospray souce in the positive ion mode. The MS analysis was performed with one full MS scan followed by three MS/MS scans on the most intense ions from the MS spectrum with the dynamic exclusion settings: repeat count 2, repeat duration 30s, exclusion duration 90s. Data were acquired in data-dependent mode using Xcalibur software.Ten raw datasets from LC-MS/MS were searched against the predicted silkworm database by Xia.et al which consists of 21312 silkworm proteins.The searching was carried out with the Turbo SEQUEST(Bioworks version 3.2, Thermo Electron).
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-07-28_00:37:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000090
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	qiying yi
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	iodoacetamide - site C: 57.021464; iodoacetamide - site C; 1-thioglycine (C-terminal): 15.977156; 1-thioglycine (C-terminal)
Instrument	LTQ Orbitrap; instrument model: LTQ-Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-10 04:34:43	ID requested
1	2014-03-26 07:43:56	announced
2	2014-07-25 08:05:07	announced	Updated project metadata.
⏵ 3	2014-07-28 00:37:47	announced	Updated project metadata.

Yi Q, Zhao P, Wang X, Zou Y, Zhong X, Wang C, Xiang Z, Xia QY, Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process. Proteomics, 13(17):2657-63(2013) [pubmed]

submitter keyword: silkworm,anterior silk gland,shotgun

qiying yi
contact affiliation	State key laboratory of silkworm genome biology
contact email	yiqiying_2011@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/03/PXD000090

PRIDE project URI

• PRIDE
  1. PXD000090
     1. Label: PRIDE project
     2. Name: Bombyx mori anterior silk gland LC-MS/MS