PXD000084

PXD000084 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Honey Bee Proteome Atlas
Description	Complex organisms are composed of organs and tissues which evolved to their present state as they function together to improve an organism's overall reproductive fitness. Studies of individual organs help us understand their basic functions but this reductionist approach misses the larger context of the whole organism. This problem can be circumvented if all the organs in an organism were comprehensively studied by the same methodology and analyzed together. Using honey bees (Apis mellifera L.) as a model system,we report here the first whole proteome of a complex organism,measuring 29 different tissue types among the three honey bee castes: queen,drone,and worker. The data reveal that,e.g.,workers have a heightened capacity to deal with environmental toxins and queens have a far more robust immune system than their nestmates. Most intriguingly,our analysis reveals the path by which organs of complex organisms probably evolved.Raw data were processed by MaxQuant (v1.2.0.13) using default parameters, plus lysine and N-terminal dimethylation for three isotopologues for relative quantitation. Data were searched against the Apis Official Gene Set version 2 with common contaminants, containing a total of 22037 sequences. Normalized intensity ratios were used for relative quantitation.
HostingRepository	PRIDE
AnnounceDate	2013-09-27
AnnouncementXML	Submission_2013-09-27_03:27:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Queenie Chan
SpeciesList	scientific name: Apis mellifera ligustica (Common honeybee) (Italian honeybee); NCBI TaxID: 7469;
ModificationList	iodoacetamide derivatized residue; monohydroxylated residue; acetylated residue; dimethylated residue; 4x(2)H labeled dimethylated residue; 2x(13)C: 4x(2)H labeled dimethylated residue
Instrument	LTQ Orbitrap: MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-04 01:51:42	ID requested
1	2013-09-10 03:33:52	announced
1	2014-07-25 00:45:58	announced
⏵ 2	2013-09-27 03:27:01	announced	Add reference
⏵ 2	2014-08-08 00:30:38	announced	Updated project metadata.

Publication List

Chan QW, Chan MY, Logan M, Fang Y, Higo H, Foster LJ, Honey bee protein atlas at organ-level resolution. Genome Res, 23(11):1951-60(2013) [pubmed]

Chan QW, Chan MY, Logan M, Fang Y, Higo H, Foster LJ, Honey bee protein atlas at organ-level resolution. Genome Res, 23(11):1951-60(2013) [pubmed]

Keyword List

submitter keyword: honey bee,proteome,LC-MS

submitter keyword: honey bee,proteome,LC-MS

Contact List

Queenie Chan
contact affiliation	Centre for High Throughput Biology
contact email	queeniecwt@gmail.com

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/09/PXD000084

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/09/PXD000084