PXD000065

PXD000065 is an original dataset announced via ProteomeXchange.

Title	HiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line
Description	Here we present high resolution data from two cancer cell lines. A human cell line, A431 was treated with a drug at different time points and then fractionated into its different subcellular compartments (nuclear, organellar, soluble, whole cell). Protein extracts were trypsinized, peptides separated by HiRIEF (high resolution isoelectric focusing) and analysed by LC-MS. From the mouse cell line N2A proteins were extracted and subjected to the same HiRIEF-LC-MS procedure.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2015-01-23_03:37:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Homo Sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue; iTRAQ8plex-115 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2012-11-06 05:29:06	ID requested
1	2013-09-27 15:10:12	announced
1	2013-11-21 02:16:33	announced
2	2014-07-25 00:44:03	announced	Updated project metadata.
⏵ 3	2015-01-23 03:37:57	announced	Updated project metadata.

Branca RM, Orre LM, Johansson HJ, Granholm V, Huss M, P, é, rez-Bercoff Å, Forshed J, K, ä, ll L, Lehti, ö J, HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics. Nat Methods, 11(1):59-62(2014) [pubmed]

submitter keyword: A431,N2A,human,mouse,LC-MS,HiRIEF,isoelectric focusing,cancer

Rui Branca
contact affiliation	Karolinska Institutet
contact email	rui.mamede-branca@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/09/PXD000065

PRIDE project URI

• PRIDE
  1. PXD000065
     1. Label: PRIDE project
     2. Name: HiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line