PXD000057

PXD000057 is an original dataset announced via ProteomeXchange.

Title	MS characterization of spidroin-1
Description	For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 10) followed by NanoLC and mass spectrometry analysis using collision-induced dissociation (CID) and electron-transfer dissociation (ETD) for fragmentation. MASCOT searches were done by using the MASCOT 2.2.06 (Matrix Science, London, UK) against database for protein identification and post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:43:21.510.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000057
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	José Roberto Aparecido dos Santos Pinto
SpeciesList	scientific name: Nephila clavipes (Golden silk orbweaver); NCBI TaxID: 6915;
ModificationList	phosphorylated residue; monohydroxylated residue; O-phospho-L-serine; O4'-phospho-L-tyrosine; deamidated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics HCT Series; instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2012-10-19 04:43:11	ID requested
⏵ 1	2022-02-22 03:43:22	announced

dos Santos-Pinto JR, Arcuri HA, Priewalder H, Salles HC, Palma MS, Lubec G, Structural Model for the Spider Silk Protein Spidroin-1. J Proteome Res, 14(9):3859-70(2015) [pubmed]

submitter keyword: Nephila sp., major ampullate silk, spidroin-1, post-translational modifications, phosphorylation, NanoLC-ESI-CID/ETD-MS/MS

José Roberto Aparecido dos Santos Pinto
contact affiliation	Department of Pediatrics, Medical University of Vienna, Vienna, Austria and Center of the Study of Social Insects, Department of Biology, São Paulo State University, Rio Claro, SP, Brazil.
contact email	jrbio08@gmail.com
lab head
José Roberto Aparecido dos Santos Pinto
contact affiliation	Department of Pediatrics, Medical University of Vienna, Vienna, Austria and Center of the Study of Social Insects, Department of Biology, São Paulo State University, Rio Claro, SP, Brazil.
contact email	jrbio08@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD000057

PRIDE project URI

• PRIDE
  1. PXD000057
     1. Label: PRIDE project
     2. Name: MS characterization of spidroin-1