PXD000052

PXD000052 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SLP76 interactome in resting and activated primary mast cells
Description	We used a knock-in (KI) mouse expressing SLP-76 with a C-terminal One-Strep-Tag (OST). Such a system allowed the replacement in the genome of SLP-76 by its tagged version and its expression in physiological amounts. Then, by combining high affinity purification and label-free quantitative proteomics, we report here the first description of the SLP76 interactome in resting and activated primary cultured mast cells derived from mice expressing an OST at the C-terminus of SLP-76 (Slp76OST/OST mice).
HostingRepository	PRIDE
AnnounceDate	2013-08-28
AnnouncementXML	Submission_2013-08-28_06:27:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hesse Anne-Marie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2012-10-16 05:23:40	ID requested
1	2013-08-28 06:24:10	announced
1	2014-07-25 00:41:34	announced
⏵ 2	2013-08-28 06:27:18	announced	Add reference

Publication List

Bounab Y, Hesse AM, Iannascoli B, Grieco L, Cout, é Y, Niarakis A, Roncagalli R, Lie E, Lam KP, Demangel C, Thieffry D, Garin J, Malissen B, Da, ë, ron M, Proteomic analysis of the SH2 domain-containing leukocyte protein of 76 kDa (SLP76) interactome in resting and activated primary mast cells [corrected]. Mol Cell Proteomics, 12(10):2874-89(2013) [pubmed]

Bounab Y, Hesse AM, Iannascoli B, Grieco L, Cout, é Y, Niarakis A, Roncagalli R, Lie E, Lam KP, Demangel C, Thieffry D, Garin J, Malissen B, Da, ë, ron M, Proteomic analysis of the SH2 domain-containing leukocyte protein of 76 kDa (SLP76) interactome in resting and activated primary mast cells [corrected]. Mol Cell Proteomics, 12(10):2874-89(2013) [pubmed]

Keyword List

submitter keyword: interactomics, SLP76, quantitative mass spectrometry

submitter keyword: interactomics, SLP76, quantitative mass spectrometry

Contact List

Hesse Anne-Marie
contact affiliation	Laboratoire d'Etude de la Dynamique des Proteomes
contact email	anne-marie.hesse@cea.fr

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/08/PXD000052

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/08/PXD000052