PXD000044
PXD000044 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stable isotope labeling with amino acids in mouse xenografts. |
| Description | This dataset corresponds to a SILAC analysis in mouse xenografts and in cell culture. We analysed signalling in tumors by means of a method based on stable isotope labeling with amino acids of xenograft tumors by the addition of [(13)C(6)]-lysine into mouse food, and also using a typical SILAC analysis in cell culture. This dataset includes 2 groups of analysis: one, with maxquant file tagged SILAX, corresponding to the xenograft analysis, the other one to SILAC analysis in cell culture. Data were acquired on an LTQ-Orbitrap XL mass spectrometer coupled with an Ultimate 3000 HPLC. Analysis was performed using MaxQuant software (version 1.1.1.36). All MS/MS spectra were searched using Andromeda against a database consisting of a combination of Homo sapiens and Mus musculus CPS databases and 250 classical contaminants. Search was performed allowing the following variable modifications: Oxidation (M) and Phospho (STY). FDR was set at 0.01 for peptides and proteins, and the minimal peptide length was six. Quantification was also performed using standard parameters; we considered only proteins with at least two identified/quantified peptides. |
| HostingRepository | PRIDE |
| AnnounceDate | 2013-01-17Z |
| AnnouncementXML | Submission_2013-01-17_02:38:42.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Serge Urbach |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2012-10-02 02:33:20 | ID requested | |
| ⏵ 1 | 2013-01-17 02:38:42 | announced | |
| ⏵ 1 | 2014-07-25 00:40:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Human, mouse, xenografts, LC MSMS, SILAC |
Contact List
| Serge Urbach | |
|---|---|
| contact affiliation | Functional Proteomics Platform |
| contact email | serge.urbach@igf.cnrs.fr |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/01/PXD000044 |
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