PXD000038

PXD000038 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of wound healing in porcine subcutaneous fatty tissue postmonopolar electrosurgery and harmonic blade incisions.
Description	Two female pigs weighing 40-55 kg were used. Each animal had four 4-cm midline caudal abdominal incisions created with the test devices. The test devices were monopolar electrosurgery (Electrocautery Pencil, ConMed, Utica NY) and Harmonic blade (HK105, Ethicon Endo-Surgery, Cincinnati OH). Each animal had two Harmonic Blade incisions and two Electrosurgery incisions for a total of 8 incisions. The incisions were created by marking the site on the ventral aspect of the abdomen and identified by level from cranial to caudal, as incision A, B, C and D respectively. The incision was started with a scalpel blade through the skin (epidermis and dermis). Each incision was deepened through the subcutaneous tissues down to the linea alba using the Harmonic at power level 5 for cutting tissue and power level 3 for coagulating bleeders, or electrosurgery set at 40 Watts for cutting and Blend 2/Spray for coagulation. The linea alba was not incised. Closure of the incision was accomplished by apposing the skin with 3-0 nylon in a simple interrupted pattern. Dermabond was then applied to seal the epidermis. No bandages were applied to the incisions. After three days, incision sites were removed en bloc from the abdominal wall. The tissue block was sliced perpendicular to the surgically incision to create slabs 4-6 mm thick. The slab was trimmed to remove the skin from the top and muscle from the bottom of the sample. The side walls were trimmed to approximately 4 mm from the surgical incision leaving a rectangular block of subcutaneous fatty tissue. Four to six samples were taken from each incision. Animals were then euthanized. Samples for genomics and proteomics analysis were placed in sterile DNAse-, RNAse-, protein-free Eppendorf tubes, flash frozen in liquid nitrogen, held on dry ice throughout tissue collection, and then stored at -80 C. Tissue samples were also collected in 10% neutral buffered formalin for histopathology analysis.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:46:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ken Pendarvis
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LCQ Deca XP Plus

Revision	Datetime	Status	ChangeLog Entry
0	2012-09-26 02:25:47	ID requested
1	2014-04-15 04:23:41	announced
⏵ 2	2014-07-24 03:46:38	announced	Updated project metadata.

Nanduri B, Pendarvis K, Shack LA, Kumar R, Clymer JW, Korvick DL, Burgess SC, Ultrasonic incisions produce less inflammatory mediator response during early healing than electrosurgical incisions. PLoS One, 8(9):e73032(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: Pig, Sus, scrofa, LCQ, healing, wound, scalpel

Ken Pendarvis
contact affiliation	Veterinary Science and Microbiology
contact email	jkpendarvis@email.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000038
     1. Label: PRIDE project
     2. Name: Proteomics analysis of wound healing in porcine subcutaneous fatty tissue postmonopolar electrosurgery and harmonic blade incisions.