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PXD000030

PXD000030 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnnexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells
DescriptionIn the present study, we used a functional proteomic approach to identify Annexin A1 (Anxa1) interacting proteins in the Philadelphia-positive KCL22 cell line. We focused on Anxa1 because it is one of the major proteins upregulated in imatinib-sensitive KCL22S cells versus imatinib-resistant KCL22R. Our proteomic strategy revealed 21 interactors. Bioinformatic analysis showed that most of these proteins are involved in cell death processes. Among the proteins identified, we studied the interaction of Anxa1 with two phosphatases, Shp1 and Shp2, which were recently identified as biomarkers of imatinib sensitivity in patients affected by chronic myeloid leukemia. Our data open new perspectives in the search for annexin-mediated signaling pathways and may shed light on mechanisms of resistance to imatinib that are unrelated to Bcr-Abl activity. For data analysis, we used the MASCOT software (version 2.4) Peptide Mass Fingerprinting search program (http://www.matrixscience.com) selecting NCBInr aug2010 database (11592891 sequences) (http://www.ncbi. nlm.nih.gov), and the following six parameters: specificity of the proteolytic enzyme used for hydrolysis (trypsin); up to 1 missed cleavage; cysteines such as S-carbamidomethylcysteines; unmodified N- and C-terminal ends; unmodified and oxidized methionines; putative Gln-induced pyroGlu formation; a precursor peptide maximum mass tolerance of 400 ppm, and a maximum fragment mass tolerance of 0.6 Da. Using the probability-based Mowse score, the ion score is 10 x Log(p), p being the probability that an eventual match is a random occurrence. All MS/MS spectra with a MASCOT score of or higher than 41 (p =< 0.05) had a good S/N, so the interpretation of the data was unambiguous. MS/MS spectra of peptides that had a MASCOT score below 41 were inspected manually and included in the statistics only in the presence of at least four continuous y or b ions.
HostingRepositoryPRIDE
AnnounceDate2013-08-13
AnnouncementXMLSubmission_2013-08-13_02:35:31.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000030
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterIrene Colavita
SpeciesList scientific name: Homo sapiens (human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deaminated residue
Instrumentmass resolution
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-09-19 05:47:59ID requested
12013-08-13 02:24:06announced
12014-07-28 00:22:04announced
22013-08-13 02:35:31announcedAdd reference
Publication List
Colavita I, Esposito N, Quintarelli C, Nigro E, Pane F, Ruoppolo M, Salvatore F, Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells. Proteomics, 13(16):2414-8(2013) [pubmed]
Keyword List
submitter keyword: Annexin A1, protein interaction, chronic myeloid leukemia
Contact List
Irene Colavita
contact affiliationProteomics
contact emailcolavita@ceinge.unina.it
Full Dataset Link List
Dataset FTP location
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Repository Record List
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