PXD000030 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells |
Description | In the present study, we used a functional proteomic approach to identify Annexin A1 (Anxa1) interacting proteins in the Philadelphia-positive KCL22 cell line. We focused on Anxa1 because it is one of the major proteins upregulated in imatinib-sensitive KCL22S cells versus imatinib-resistant KCL22R. Our proteomic strategy revealed 21 interactors. Bioinformatic analysis showed that most of these proteins are involved in cell death processes. Among the proteins identified, we studied the interaction of Anxa1 with two phosphatases, Shp1 and Shp2, which were recently identified as biomarkers of imatinib sensitivity in patients affected by chronic myeloid leukemia. Our data open new perspectives in the search for annexin-mediated signaling pathways and may shed light on mechanisms of resistance to imatinib that are unrelated to Bcr-Abl activity. For data analysis, we used the MASCOT software (version 2.4) Peptide Mass Fingerprinting search program (http://www.matrixscience.com) selecting NCBInr aug2010 database (11592891 sequences) (http://www.ncbi. nlm.nih.gov), and the following six parameters: specificity of the proteolytic enzyme used for hydrolysis (trypsin); up to 1 missed cleavage; cysteines such as S-carbamidomethylcysteines; unmodified N- and C-terminal ends; unmodified and oxidized methionines; putative Gln-induced pyroGlu formation; a precursor peptide maximum mass tolerance of 400 ppm, and a maximum fragment mass tolerance of 0.6 Da. Using the probability-based Mowse score, the ion score is 10 x Log(p), p being the probability that an eventual match is a random occurrence. All MS/MS spectra with a MASCOT score of or higher than 41 (p =< 0.05) had a good S/N, so the interpretation of the data was unambiguous. MS/MS spectra of peptides that had a MASCOT score below 41 were inspected manually and included in the statistics only in the presence of at least four continuous y or b ions. |
HostingRepository | PRIDE |
AnnounceDate | 2013-08-13 |
AnnouncementXML | Submission_2013-08-13_02:35:31.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000030 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Irene Colavita |
SpeciesList | scientific name: Homo sapiens (human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deaminated residue |
Instrument | mass resolution |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2012-09-19 05:47:59 | ID requested | |
1 | 2013-08-13 02:24:06 | announced | |
1 | 2014-07-28 00:22:04 | announced | |
⏵ 2 | 2013-08-13 02:35:31 | announced | Add reference |
Publication List
Colavita I, Esposito N, Quintarelli C, Nigro E, Pane F, Ruoppolo M, Salvatore F, Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells. Proteomics, 13(16):2414-8(2013) [pubmed] |
Keyword List
submitter keyword: Annexin A1, protein interaction, chronic myeloid leukemia |
Contact List
Irene Colavita |
contact affiliation | Proteomics |
contact email | colavita@ceinge.unina.it |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/08/PXD000030 |
Repository Record List
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- PRIDE
- 27259
- InstrumentRef: INSTRUMENT_1
- Label: slide F2a
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide F2a
- PublicationRef: PMID23754495
- 27262
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- Label: slide D4a
- ModificationList: deaminated residue, monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide D4a
- PublicationRef: PMID23754495
- 27253
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- Label: slide D3a
- ModificationList: deaminated residue, monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide D3a
- PublicationRef: PMID23754495
- 27263
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- Label: slide I5
- ModificationList: monohydroxylated residue, deaminated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide I5
- PublicationRef: PMID23754495
- 27260
- InstrumentRef: INSTRUMENT_1
- Label: slide F6
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue, deaminated residue,
- Name: Gel-based proteomics slide F6
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- 27251
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- Label: slide D5
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide D5
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- 27255
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- Name: Gel-based proteomics slide C5a
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- Label: slide D3b
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- Name: Gel-based proteomics slide D3b
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- Label: slide D4b
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- Name: Gel-based proteomics slide D4b
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- Label: slide D4
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- Name: Gel-based proteomics slide D4
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- 27254
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- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide C3
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- 27257
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- Name: Gel-based proteomics slide E2
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- 27256
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- Label: slide F2
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide F2
- PublicationRef: PMID23754495
- 27261
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- Label: slide G1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue,
- Name: Gel-based proteomics slide G1
- PublicationRef: PMID23754495
- 27250
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- Label: ccc
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue,
- Name: dddd
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- 27265
- InstrumentRef: INSTRUMENT_1
- Label: slide D3
- ModificationList: deaminated residue, monohydroxylated residue, iodoacetamide derivatized residue,
- Name: Gel-based proteomics slide D3
- PublicationRef: PMID23754495