PXD000023

PXD000023 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	EHEC O157:H7 Sakai vs. TW14359: Secreted and Cytosolic iTRAQ; sepD secretome analysis of TW14359
Description	Comparative analysis of EHEC O157:H7 Sakai vs. TW14359 secreted and cytosolic protein using iTRAQ and mass spectrometry on an LTQ Orbitrap. Samples were fractionated through strong cation exchange and HPLC before analsyis. For TW14359 sepD secretome analysis, strains were similarly prepared and analyzed. All samples were searched against a TW14359 specific protein database (Uniprot) coupled with randomized protein sequences.
HostingRepository	PRIDE
AnnounceDate	2013-09-06
AnnouncementXML	Submission_2013-09-06_03:38:09.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000023
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jason Morgan
SpeciesList	scientific name: Escherichia coli O157:H7 (strain TW14359 / EHEC); NCBI TaxID: 544404;
ModificationList	monohydroxylated residue: 15.994915; methylthiolated residue: 45.987721; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry: 144.102062; iodoacetamide -site C: 57.021464
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2012-09-06 08:47:03	ID requested
1	2013-09-05 10:24:53	announced
1	2014-07-28 00:20:25	announced
⏵ 2	2013-09-06 03:38:09	announced	Add reference

Publication List

Morgan JK, Vendura KW, Stevens SM, Riordan JT, RcsB determines the locus of enterocyte effacement (LEE) expression and adherence phenotype of Escherichia coli O157 : H7 spinach outbreak strain TW14359 and coordinates bicarbonate-dependent LEE activation with repression of motility. Microbiology (Reading), 159(Pt 11):2342-2353(2013) [pubmed]

Morgan JK, Vendura KW, Stevens SM, Riordan JT, RcsB determines the locus of enterocyte effacement (LEE) expression and adherence phenotype of Escherichia coli O157 : H7 spinach outbreak strain TW14359 and coordinates bicarbonate-dependent LEE activation with repression of motility. Microbiology (Reading), 159(Pt 11):2342-2353(2013) [pubmed]

Keyword List

submitter keyword: Enterohemorrhagic Escherichia coli O157:H7, Sakai, TW14359, Spinach, iTRAQ, sepD, hypersecretion, Secretome, LTQ Orbitrap, LTQ

submitter keyword: Enterohemorrhagic Escherichia coli O157:H7, Sakai, TW14359, Spinach, iTRAQ, sepD, hypersecretion, Secretome, LTQ Orbitrap, LTQ

Contact List

Jason Morgan
contact affiliation	University of South Florida - Department of Cell Biology, Microbiology, and Molecular Biology
contact email	jkmorgan@mail.usf.edu
Dr. James Riordan
contact attribute	jtriordan@usf.edu
contact affiliation	University of South Florida
contact email	jtriordan@usf.edu

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/09/PXD000023

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/09/PXD000023

Repository Record List

[ + ]

PRIDE
1. 27208
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143
2. 27203
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143
3. 27199
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC Intracellular iTRAQ TW14359 vs. Sakai
  3. ModificationList: monohydroxylated residue, methylthiolated residue,
  4. Name: EHEC Intracelluar iTRAQ
  5. PublicationRef: PMID23985143
4. 27207
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143
5. 27204
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143
6. 27202
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC Secreted iTRAQ Second Run
  3. ModificationList: Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry, monohydroxylated residue, methylthiolated residue,
  4. Name: EHEC Secreted iTRAQ
  5. PublicationRef: PMID23985143
7. 27201
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC Secreted iTRAQ
  3. ModificationList: Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry, monohydroxylated residue, methylthiolated residue,
  4. Name: EHEC Secreted iTRAQ
  5. PublicationRef: PMID23985143
8. 27205
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143
9. 27200
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC Intracellular iTRAQ TW14359 vs. Sakai - 2
  3. ModificationList: Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry, monohydroxylated residue, methylthiolated residue,
  4. Name: EHEC Intracellular iTRAQ TW14359 vs. Sakai
  5. PublicationRef: PMID23985143
10. 27206
  1. InstrumentRef: INSTRUMENT_1
  2. Label: EHEC TW14359 sepD null grlA++
  3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
  4. Name: EHEC TW14359 sepD null grlA++
  5. PublicationRef: PMID23985143