PXD000020

PXD000020 is an original dataset announced via ProteomeXchange.

Title	The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics
Description	Proteomics on B. thuringiensis CT_43 cells in GYS medium. Two biological replicate cell samples were collected at time points of 7 h, 9 h, 13 h and 22 h, respectively. The crude proteins were purified using the ReadyPrep 2-D Cleanup Kit, underwent the reductive alkylation, tryptically digested, and were labeled with 8-plex iTRAQ reagents as follows: 7 h-1, 113; 7 h-2, 114; 9 h-1, 115; 9 h-2, 116; 13 h-1, 117; 13 h-2, 118; 22 h-1, 119; and 22 h-2, 121. The labeled samples were pooled and resolved into 12 fractions, which were loaded onto LC-MSMS.
HostingRepository	PRIDE
AnnounceDate	2019-11-22
AnnouncementXML	Submission_2020-01-30_01:04:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000020
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jieping Wang
SpeciesList	scientific name: Escherichia coli O157:NM; NCBI TaxID: 626528;
ModificationList	monohydroxylated residue; iodoacetamide - site C; 2-pyrrolidone-5-carboxylic acid (Gln); iTRAQ8plex reporter+balance reagent acylated N-terminal
Instrument	TripleTOF 5600; instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2012-08-31 05:45:00	ID requested
⏵ 1	2020-01-30 01:14:14	announced
⏵ 1	2020-01-30 01:14:15	announced

Wang J, Mei H, Zheng C, Qian H, Cui C, Fu Y, Su J, Liu Z, Yu Z, He J, The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics. Mol Cell Proteomics, 12(5):1363-76(2013) [pubmed]

submitter keyword: Bacillus thuringiensis LC-MS/MS

Jin He
contact affiliation	State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PRC
contact email	wangjieping2011@163.com
lab head
Jieping Wang
contact affiliation	Huazhong Agricultural University
contact email	wangjieping2011@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000020
     1. Label: PRIDE project
     2. Name: The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics