PXD000017
PXD000017 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry |
Description | Studies of neuronal, endocrine and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). Peptide features were detected, aligned and searched against a rat SwissProt database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21,455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of RAB family and proteins involved in glutamate biosynthesis. |
HostingRepository | PRIDE |
AnnounceDate | 2012-10-03+01:00 |
AnnouncementXML | Submission_2012-10-03_04:29:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Viktoria Stelzhammer |
SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q-TOF Premier; ACQUITY UPLC; electrospray ionization; microchannel plate detector |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2012-08-17 05:50:14 | ID requested | |
1 | 2012-08-23 02:46:42 | announced | |
1 | 2014-05-21 03:32:52 | announced | |
⏵ 2 | 2012-10-03 04:29:46 | announced | missing |
⏵ 2 | 2014-07-25 00:37:04 | announced | Updated project metadata. |
Publication List
no publication |
Keyword List
submitter keyword: rat, proteome, hypothalamus |
Contact List
Viktoria Stelzhammer | |
---|---|
contact affiliation | University of Cambridge, Department of Chemical Engineering and Biotechnology |
contact email | vs308@cam.ac.uk |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2012/08/PXD000017 |