PXD000017 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry |
Description | Studies of neuronal, endocrine and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). Peptide features were detected, aligned and searched against a rat SwissProt database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21,455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of RAB family and proteins involved in glutamate biosynthesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_03:59:13.411.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Viktoria Stelzhammer |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | microchannel plate detector; Q-Tof Premier; ACQUITY UPLC; electrospray ionization |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2012-08-17 05:50:14 | ID requested | |
1 | 2012-08-23 02:46:42 | announced | |
1 | 2014-05-21 03:32:52 | announced | |
2 | 2012-10-03 04:29:46 | announced | missing |
2 | 2014-07-25 00:37:04 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 03:59:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/pmic.201100642; |
Stelzhammer V, Amess B, Martins-de-Souza D, Levin Y, Ozanne SE, Martin-Gronert MS, Urday S, Bahn S, Guest PC, Analysis of the rat hypothalamus proteome by data-independent label-free LC-MS/MS. Proteomics, 12(22):3386-92(2012) [pubmed] |
Keyword List
submitter keyword: hypothalamus,rat, proteome |
Contact List
Viktoria Stelzhammer |
contact affiliation | Chemical Engineering and Biotechnology |
contact email | vs308@cam.ac.uk |
lab head | |
Viktoria Stelzhammer |
contact affiliation | Chemical Engineering and Biotechnology |
contact email | vs308@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000017
- Label: PRIDE project
- Name: Analysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry