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PXD000017

PXD000017 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry
DescriptionStudies of neuronal, endocrine and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). Peptide features were detected, aligned and searched against a rat SwissProt database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21,455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of RAB family and proteins involved in glutamate biosynthesis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:59:13.411.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterViktoria Stelzhammer
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListiodoacetamide derivatized residue
Instrumentmicrochannel plate detector; Q-Tof Premier; ACQUITY UPLC; electrospray ionization
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-08-17 05:50:14ID requested
12012-08-23 02:46:42announced
12014-05-21 03:32:52announced
22012-10-03 04:29:46announcedmissing
22014-07-25 00:37:04announcedUpdated project metadata.
32024-10-22 03:59:14announced2024-10-22: Updated project metadata.
Publication List
10.1002/pmic.201100642;
Stelzhammer V, Amess B, Martins-de-Souza D, Levin Y, Ozanne SE, Martin-Gronert MS, Urday S, Bahn S, Guest PC, Analysis of the rat hypothalamus proteome by data-independent label-free LC-MS/MS. Proteomics, 12(22):3386-92(2012) [pubmed]
Keyword List
submitter keyword: hypothalamus,rat, proteome
Contact List
Viktoria Stelzhammer
contact affiliationChemical Engineering and Biotechnology
contact emailvs308@cam.ac.uk
lab head
Viktoria Stelzhammer
contact affiliationChemical Engineering and Biotechnology
contact emailvs308@cam.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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