PXD000017

PXD000017 is an original dataset announced via ProteomeXchange.

Title	Analysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry
Description	Studies of neuronal, endocrine and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). Peptide features were detected, aligned and searched against a rat SwissProt database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21,455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of RAB family and proteins involved in glutamate biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:59:13.411.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viktoria Stelzhammer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	microchannel plate detector; Q-Tof Premier; ACQUITY UPLC; electrospray ionization

Revision	Datetime	Status	ChangeLog Entry
0	2012-08-17 05:50:14	ID requested
1	2012-08-23 02:46:42	announced
1	2014-05-21 03:32:52	announced
2	2012-10-03 04:29:46	announced	missing
2	2014-07-25 00:37:04	announced	Updated project metadata.
⏵ 3	2024-10-22 03:59:14	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201100642;

Stelzhammer V, Amess B, Martins-de-Souza D, Levin Y, Ozanne SE, Martin-Gronert MS, Urday S, Bahn S, Guest PC, Analysis of the rat hypothalamus proteome by data-independent label-free LC-MS/MS. Proteomics, 12(22):3386-92(2012) [pubmed]

submitter keyword: hypothalamus,rat, proteome

Viktoria Stelzhammer
contact affiliation	Chemical Engineering and Biotechnology
contact email	vs308@cam.ac.uk
lab head
Viktoria Stelzhammer
contact affiliation	Chemical Engineering and Biotechnology
contact email	vs308@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2012/08/PXD000017

PRIDE project URI

• PRIDE
  1. PXD000017
     1. Label: PRIDE project
     2. Name: Analysis of the rat hypothalamus proteome by data independent label-free liquid chromatography tandem mass spectrometry