PXD000014
PXD000014 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | human 293 cells PEDF LC-MS/MS |
Description | Experiment was performed on 293 cells, details are presented in publication: Adenoviral E4 gene stimulates secretion of PEDF that maintains long-term survival of human glomerulus-derived endothelial cells Marina Jerebtsova‡§*, Namita Kumari‖, Yuri Obuhkov‖, and Sergei Nekhai‖†* (to be published in Molecular and Cellular Proteomics), please see more details in paper Workflow HPLC on PicoFrit C18 column, gradient 60 min from 2% to 30% ACN Nanospray 2 kV, flow rate 300 nL/min LTQ Orbitrap MS. Data dependent scan FT MS + 3 FT MS/MS. A blank water run was performed after each sample run Each 24 hours setup performance was tested on Apomyoglobin sample, 12 fmol. Coverage was 39-79 % Search is made by SEQUEST on human database |
HostingRepository | PRIDE |
AnnounceDate | 2012-10-03+01:00 |
AnnouncementXML | Submission_2012-10-03_04:25:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuri Obukhov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2012-08-08 01:23:24 | ID requested | |
1 | 2012-08-08 08:53:13 | announced | |
1 | 2014-07-25 00:36:44 | announced | |
2 | 2012-08-16 03:31:30 | announced | missing |
⏵ 3 | 2012-10-03 04:25:02 | announced | missing |
Publication List
no publication |
Keyword List
submitter keyword: angiogenesis, endothelium, pigmental epithelium derived factor, adenovirus, senecence |
Contact List
Yuri Obukhov | |
---|---|
contact affiliation | Department of Medicine |
contact email | oboukhovi.louri@howard.edu |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2012/08/PXD000014 |