<<< Full experiment listing

PXD000014

PXD000014 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlehuman 293 cells PEDF LC-MS/MS
DescriptionExperiment was performed on 293 cells, details are presented in publication: Adenoviral E4 gene stimulates secretion of PEDF that maintains long-term survival of human glomerulus-derived endothelial cells Marina Jerebtsova‡§*, Namita Kumari‖, Yuri Obuhkov‖, and Sergei Nekhai‖†* (to be published in Molecular and Cellular Proteomics), please see more details in paper Workflow HPLC on PicoFrit C18 column, gradient 60 min from 2% to 30% ACN Nanospray 2 kV, flow rate 300 nL/min LTQ Orbitrap MS. Data dependent scan FT MS + 3 FT MS/MS. A blank water run was performed after each sample run Each 24 hours setup performance was tested on Apomyoglobin sample, 12 fmol. Coverage was 39-79 % Search is made by SEQUEST on human database
HostingRepositoryPRIDE
AnnounceDate2012-10-03+01:00
AnnouncementXMLSubmission_2012-10-03_04:25:02.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuri Obukhov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-08-08 01:23:24ID requested
12012-08-08 08:53:13announced
12014-07-25 00:36:44announced
22012-08-16 03:31:30announcedmissing
32012-10-03 04:25:02announcedmissing
Publication List
no publication
Keyword List
submitter keyword: angiogenesis, endothelium, pigmental epithelium derived factor, adenovirus, senecence
Contact List
Yuri Obukhov
contact affiliationDepartment of Medicine
contact emailoboukhovi.louri@howard.edu
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2012/08/PXD000014