PXD000012

PXD000012 is an original dataset announced via ProteomeXchange.

Title	Resveratrol-induced changes of the human adipocyte secretion profile.
Description	Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV) a natural polyphenolic compound mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects are beneficial to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified.
HostingRepository	PRIDE
AnnounceDate	2012-10-03+01:00
AnnouncementXML	Submission_2012-10-26_12:59:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000012
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean-Paul Noben
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue: 15.994915; iodoacetamide -site C: 57.021464
Instrument	Ion Trap

Revision	Datetime	Status	ChangeLog Entry
0	2012-08-03 01:27:33	ID requested
1	2012-08-31 05:25:00	announced
1	2014-07-28 00:18:49	announced
2	2012-09-03 03:50:54	announced	Add reference
2	2017-06-21 00:30:21	announced	Updated project metadata.
3	2012-10-03 02:40:37	announced	Add reference
3	2017-10-24 00:06:11	announced	Updated project metadata.
⏵ 4	2012-10-26 12:59:33	announced	Add reference

Rosenow A, Noben JP, Jocken J, Kallendrusch S, Fischer-Posovszky P, Mariman EC, Renes J, Resveratrol-induced changes of the human adipocyte secretion profile. J Proteome Res, 11(9):4733-43(2012) [pubmed]

submitter keyword: human adipocytes, RSV, adipokines, lipolysis, 2-DE LC-MS/MS

Anja Rosenow
contact affiliation	Department of Human Biology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. Phone: 0031 43 38 81509. Fax: 0031 43 36 70976.
contact email	a.rosenow@maastrichtuniversity.nl
Jean-Paul Noben
contact affiliation	FBI
contact email	jeanpaul.noben@uhasselt.be

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• PRIDE
  1. 27081
     1. InstrumentRef: LCQ_Duo
     2. Label: RSVAR81
     3. ModificationList: monohydroxylated residue, iodoacetamide -site C,
     4. Name: Resveratrol-induced changes of the human adipocyte secretion profile.
     5. PublicationRef: PMID22905912