PXD000006

PXD000006 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of 15d-PGJ2 target proteins
Description	15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) is an endogenous anti-inflammatory lipid derived from PGD2. One potential mechanism for its activity is the covalent modification of cellular proteins, via a reactive alpha,beta-unsaturated carbonyl group in its cyclopentenone ring, which in turn alters protein function. In order to identify the target proteins covalently modified by 15d-PGJ2 in human aortic endothelial cell (EC), EC were treated with biotinylated-15d-PGJ2, the modified proteins extracted by Neutravidin affinity-purification and the proteins were identified by LTQ Orbitrap mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_08:39:05.890.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000006
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Simone Marcone
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2012-07-10 08:36:54	ID requested
1	2015-04-27 04:11:10	announced
2	2015-04-27 06:01:27	announced	Updated publication details.
3	2015-04-28 00:30:29	announced	Updated project metadata.
⏵ 4	2024-10-07 08:39:06	announced	2024-10-07: Updated project metadata.

Publication List

10.1002/pmic.201200289;
Marcone S, Fitzgerald DJ, Proteomic identification of the candidate target proteins of 15-deoxy-delta12,14-prostaglandin J2. Proteomics, 13(14):2135-9(2013) [pubmed]
10.6019/PXD000006;

10.1002/pmic.201200289;

Marcone S, Fitzgerald DJ, Proteomic identification of the candidate target proteins of 15-deoxy-delta12,14-prostaglandin J2. Proteomics, 13(14):2135-9(2013) [pubmed]

10.6019/PXD000006;

Keyword List

curator keyword: Biomedical
submitter keyword: covalent modification of proteins,15d-PGJ2, endothelial cell

curator keyword: Biomedical

submitter keyword: covalent modification of proteins,15d-PGJ2, endothelial cell

Contact List

Simone Marcone
contact affiliation	University College Dublin
contact email	simone.marcone@ucd.ie
lab head
Simone Marcone
contact affiliation	University College Dublin
contact email	simone.marcone@ucd.ie
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD000006

PRIDE project URI

Repository Record List

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