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PXD000005

PXD000005 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic screen reveals Fbw7 as a modulator of the NF-kB pathway
DescriptionProteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS
HostingRepositoryPRIDE
AnnounceDate2023-11-01
AnnouncementXMLSubmission_2023-11-01_09:15:59.847.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuiBranca
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-06-29 03:03:15ID requested
12012-06-29 03:50:02announced
12014-07-24 08:12:15announced
22012-06-29 04:33:50announcedmissing
32012-06-29 04:36:58announcedmissing
42012-08-13 01:54:50announcedmissing
52012-10-03 02:27:01announcedmissing
62012-10-26 13:01:14announcedmissing
72023-11-01 09:05:00announced2023-11-01: Updated project metadata.
82023-11-01 09:16:00announced2023-11-01: Updated project metadata.
Publication List
Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Ari, ë, s I, Nilsson P, Den Boer ML, Pokrovskaja K, Grand, é, r D, Xiao G, Rocha S, Lehti, ö J, Sangfelt O, B pathway. Nat Commun, 3():976(2012) [pubmed]
Keyword List
submitter keyword: LC-MSMS Orbitrap human colon epithelium Fbw7 HiRIEF
Contact List
Rui Branca
contact affiliationClinical Proteomics Unit, Dep. of Oncology-Pathology
contact emailrui.mamede-branca@ki.se
lab head
RuiBranca
contact affiliationClinical Proteomics Unit, Dep. of Oncology-Pathology
contact emailrui.mamede-branca@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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