PXD000005 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway |
Description | Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-01 |
AnnouncementXML | Submission_2023-11-01_09:15:59.847.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | RuiBranca |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2012-06-29 03:03:15 | ID requested | |
1 | 2012-06-29 03:50:02 | announced | |
1 | 2014-07-24 08:12:15 | announced | |
2 | 2012-06-29 04:33:50 | announced | missing |
3 | 2012-06-29 04:36:58 | announced | missing |
4 | 2012-08-13 01:54:50 | announced | missing |
5 | 2012-10-03 02:27:01 | announced | missing |
6 | 2012-10-26 13:01:14 | announced | missing |
7 | 2023-11-01 09:05:00 | announced | 2023-11-01: Updated project metadata. |
⏵ 8 | 2023-11-01 09:16:00 | announced | 2023-11-01: Updated project metadata. |
Publication List
Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Ari, ë, s I, Nilsson P, Den Boer ML, Pokrovskaja K, Grand, é, r D, Xiao G, Rocha S, Lehti, ö J, Sangfelt O, B pathway. Nat Commun, 3():976(2012) [pubmed] |
Keyword List
submitter keyword: LC-MSMS Orbitrap human colon epithelium Fbw7 HiRIEF |
Contact List
Rui Branca |
contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
contact email | rui.mamede-branca@ki.se |
lab head | |
RuiBranca |
contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
contact email | rui.mamede-branca@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2012/06/PXD000005 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000005
- Label: PRIDE project
- Name: Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway