PXD028948 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | C. difficle p-cresol production |
Description | To identify the cellular localisation of the HpdBCA decarboxylase complex, a plasmid based HpdB-SNAP-tag translational fusion was constructed using a C. difficile compatible plasmid (pMTL84151) under control of the hpdBCA promoter. The hpdB coding sequence omitting the stop codon, was fused via a linker to a SNAP-tag (Table 1). Confirmation of HpdB linked to the SNAP-tag was carried out via western blot, and mass spectrometry, in which we identified six peptides, four with 100% identity and two with 99% identity unique to HpdB. Localisation of HpdB was visualised by confocal microscopy in 630Δerm and the p-cresol deficient mutant (hpdC::CT) (carrying the HpdB-SNAP-tag fusion (PhpdB-CDS-SNAP), in the presence and absence of p-HPA) to induce HpdBCA production. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-02 |
AnnouncementXML | Submission_2021-11-02_04:16:20.249.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Steven Lynham |
SpeciesList | scientific name: Clostridioides difficile ATCC 43255; NCBI TaxID: 499175; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-05 02:47:48 | ID requested | |
⏵ 1 | 2021-11-02 04:16:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HpdBCA decarboxylase |
Contact List
Dr Lisa Dawson |
contact affiliation | Infection Biology – LSHTM, Keppel street, WC1E 7HT |
contact email | lisa.dawson@lshtm.ac.uk |
lab head | |
Steven Lynham |
contact affiliation | King's College London |
contact email | steven.lynham@kcl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028948
- Label: PRIDE project
- Name: C. difficle p-cresol production