PXD013024

PXD013024 is an original dataset announced via ProteomeXchange.

Title	HiRIEF-LC-MS based proteomics on Small Intestinal Neuroendocrine Tumors (SI-NET) and SI-NET cell lines
Description	Associated to PXD009877 which contains Part I and Part II. Part III – Pevonedistat/MLN4924 and Bortezomib treatment of the SI-NET (small intestinal – neuroendocrine tumor) cell line CNDT2. High Resolution Isoelectric Focusing (HiRIEF) LC-MS and relative quantification by TMT 10-plex was used to analyze cellular response to the proteasome inhibitor Bortezomib alone or in combination with the neddylation inhibitor pevonedistat (MLN4924) at 12h after treatment in the CNDT2 cell line. The data was obtained from one TMT 10-plex experiment which included 4 untreated controls (TMT channels 126, 127N, 127C and 128N), 3 samples treated with 500 nM Bortezomib for 12 hours (TMT channels 128C, 129N and 129C) as well as 3 samples treated with both Bortezomib and Pevonedistat at 500 nM respectively for 12 hours (TMT channels 130N, 130C and 131).
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2021-06-10_02:39:34.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mattias Vesterlund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-08 08:09:41	ID requested
⏵ 1	2021-06-10 02:39:35	announced

Fotouhi O, Kjellin H, Juhlin CC, Pan Y, Vesterlund M, Ghaderi M, Yousef A, Andersson-Sand H, Kharaziha P, Caramuta S, Kjellman M, Zedenius J, Larsson C, Orre LM, Proteomics identifies neddylation as a potential therapy target in small intestinal neuroendocrine tumors. Oncogene, 38(43):6881-6897(2019) [pubmed]

submitter keyword: Human, Cell line, Neuro endocrine Tumor, Bortezomib, Pevonedistat

Janne Lehtiö
contact affiliation	Dept. Oncology Pathology, Science for Life Laboratory, Karolinska Institutet, Sweden
contact email	janne.lehtio@ki.se
lab head
Mattias Vesterlund
contact affiliation	Karolinska Institutet
contact email	mattias.vesterlund@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD013024

PRIDE project URI

• PRIDE
  1. PXD013024
     1. Label: PRIDE project
     2. Name: HiRIEF-LC-MS based proteomics on Small Intestinal Neuroendocrine Tumors (SI-NET) and SI-NET cell lines